Dataset Open Access

Single particle tracking data for "Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD"

Lu, Huasong; Yu, Dan; Hansen, Anders S.; Ganguly, Sourav; Liu, Rongdiao; Heckert, Alec; Darzacq, Xavier; Zhou, Qiang

Experimental single-particle tracking (SPT) data supporting "Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD"

This dataset contains all the raw SPT data reported in "Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD" in the form of SPT trajectories. The SPT trajectories are provided in two different formats for convenience: a CSV format and a Matlab format. Both formats are readable by Spot-On: https://spoton.berkeley.edu/

The SPT data contains "fast tracking" spaSPT data (Figure 2d) and this data was analyzed using the Matlab version of Spot-On which can be found and downloaded at: https://gitlab.com/tjian-darzacq-lab/spot-on-matlab

The SPT data also contains "slow tracking" SPT data (Figure 2e).

Full details about the Matlab and CSV formats are provided in the ReadMe files in the associated zip files.

Please see the associated manuscript for a detailed description of how the data was acquired and analyzed. For questions about the data please contact Anders Sejr Hansen at anders.sejr.hansen {at} berkeley {dot} edu.

This work was supported by the Public Health Service grant R01AI041757 from the National Institutes of Health to Q.Z; the California Institute of Regenerative Medicine grant LA1-08013 and, National Institutes of Health grants UO1-EB021236 and U54-DK107980 to X.D.; The Siebel Stem Cell Institute to A.S.H.
Files (139.0 MB)
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SPT_data_CSV.zip
md5:a320bb96662c84a5c8a023209179f6c1
64.6 MB Download
SPT_data_MAT.zip
md5:5dbab6b3e6a197d4ef4e320b73732e10
74.4 MB Download
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