January 19, 2017 Journal article Open Access

Luca Piemontese; Filippo Maria Perna; Antonio Logrieco; Vito Capriati; Michele Solfrizzo

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{
  "description": "<p>An unprecedented, environmentally friendly, and faster method for the determination of<br>\nOchratoxin A (OTA) (a mycotoxin produced by several species of Aspergillus and Penicillium and<br>\nlargely widespread in nature, in wheat and derived products) has, for the first time, been set up and<br>\nvalidated using choline chloride (ChCl)-based deep eutectic solvents (DESs) (e.g., ChCl/glycerol<br>\n(1:2) and ChCl/ urea (1:2) up to 40% (w/w) water) as privileged, green, and biodegradable extraction<br>\nsolvents. This also reduces worker exposure to toxic chemicals. Results are comparable to those<br>\nobtained using conventional, hazardous and volatile organic solvents (VOCs) typical of the standard<br>\nand official methods. OTA recovery from spiked durum wheat samples, in particular, was to up to<br>\n89% versus 93% using the traditional acetonitrile-water mixture with a repeatability of the results<br>\n(RSDr) of 7%. Compatibility of the DES mixture with the antibodies of the immunoaffinity column<br>\nwas excellent as it was able to retain up to 96% of the OTA. Recovery and repeatability for durum<br>\nwheat, bread crumbs, and biscuits proved to be within the specifications required by the current<br>\nEuropean Commission (EC) regulation. Good results in terms of accuracy and precision were<br>\nachieved with mean recoveries between 70% (durum wheat) and 88% (bread crumbs) and an RSDr<br>\nbetween 2% (biscuits) and 7% (bread).</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Ispa-Cnr", 
      "@type": "Person", 
      "name": "Luca Piemontese"
    }, 
    {
      "affiliation": "Dipartimento di Farmacia\u2013Scienze del Farmaco, Universit\u00e0 degli Studi di Bari \"Aldo Moro\"", 
      "@type": "Person", 
      "name": "Filippo Maria Perna"
    }, 
    {
      "affiliation": "Ispa-Cnr", 
      "@type": "Person", 
      "name": "Antonio Logrieco"
    }, 
    {
      "affiliation": "Dipartimento di Farmacia\u2013Scienze del Farmaco, Universit\u00e0 degli Studi di Bari \"Aldo Moro\"", 
      "@type": "Person", 
      "name": "Vito Capriati"
    }, 
    {
      "affiliation": "Ispa-Cnr", 
      "@type": "Person", 
      "name": "Michele Solfrizzo"
    }
  ], 
  "headline": "Deep Eutectic Solvents as Novel and Effective Extraction Media for Quantitative Determination of Ochratoxin A in Wheat and Derived Product", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2017-01-19", 
  "url": "https://zenodo.org/record/252327", 
  "keywords": [
    "deep eutectic solvents; Ochratoxin A; food chemistry; analytical method; green solvents"
  ], 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.3390/molecules22010121", 
  "@id": "https://doi.org/10.3390/molecules22010121", 
  "@type": "ScholarlyArticle", 
  "name": "Deep Eutectic Solvents as Novel and Effective Extraction Media for Quantitative Determination of Ochratoxin A in Wheat and Derived Product"
}