10.3390/toxins9020045
https://zenodo.org/records/264018
oai:zenodo.org:264018
Vania C. Liuzzi
Vania C. Liuzzi
Institute of Sciences of Food Production, CNR, 70126 Bari, Italy
Valentina Mirabelli
Valentina Mirabelli
Institute of Crystallography, CNR, 70126 Bari, Italy
Maria Teresa Cimmarusti
Maria Teresa Cimmarusti
Institute of Sciences of Food Production, CNR, 70126 Bari, Italy, Department of Economics, University of Foggia, 71121 Foggia, Italy
Miriam Haidukowski
Miriam Haidukowski
Institute of Sciences of Food Production, CNR, 70126 Bari, Italy
John F. Leslie
John F. Leslie
Department of Plant Pathology, Kansas State University, Manhattan, 66506 KS, USA
Antonio F. Logrieco
Antonio F. Logrieco
Institute of Sciences of Food Production, CNR, 70126 Bari, Italy
Rocco Caliandro
Rocco Caliandro
Institute of Crystallography, CNR, 70126 Bari, Italy
Francesca Fanelli
Francesca Fanelli
Institute of Sciences of Food Production, CNR, 70126 Bari, Italy
Giuseppina Mulè
Giuseppina Mulè
Institute of Sciences of Food Production, CNR, 70126 Bari, Italy
Enniatin and Beauvericin Biosynthesis in Fusarium Species: Production Profiles and Structural Determinant Prediction
Zenodo
2017
enniatin; beauvericin; ESYN1; mycotoxins; Fusarium; homology modelling; multivariate analysis; backbone angles
2017-01-25
https://zenodo.org/communities/eu
Creative Commons Attribution 4.0 International
Members of the fungal genus Fusarium can produce numerous secondary metabolites, including the nonribosomal mycotoxins beauvericin (BEA) and enniatins (ENNs). Both mycotoxins are synthesized by the multifunctional enzyme enniatin synthetase (ESYN1) that contains both peptide synthetase and S-adenosyl-l-methionine-dependent N-methyltransferase activities. Several Fusarium species can produce ENNs, BEA or both, but the mechanism(s) enabling these differential metabolic profiles is unknown. In this study, we analyzed the primary structure of ESYN1 by sequencing esyn1 transcripts from different Fusarium species. We measured ENNs and BEA production by ultra-performance liquid chromatography coupled with photodiode array and Acquity QDa mass detector (UPLC-PDA-QDa) analyses. We predicted protein structures, compared the predictions by multivariate analysis methods and found a striking correlation between BEA/ENN-producing profiles and ESYN1 three-dimensional structures. Structural differences in the β strand's Asn789-Ala793 and His797-Asp802 portions of the amino acid adenylation domain can be used to distinguish BEA/ENN-producing Fusarium isolates from those that produce only ENN.
European Commission
10.13039/501100000780
678781
Integrated and innovative key actions for mycotoxin management in the food and feed chain