3381096
doi
10.1371/journal.pone.0220743
oai:zenodo.org:3381096
user-fhtw
Xavier Monforte Vila
University of Applied Sciences Technikum Wien
Elias Salzer
University of Applied Sciences Technikum Wien
Andreas Teuschl
University of Applied Sciences Technikum Wien
Lachmi Jenndahl
VERIGRAFT AB
Niklas Bergh
University of Gothenburg
Per Fogelstrand
University of Gothenburg
Effect of fluid dynamics on decellularization efficacy and mechanical properties of blood vessels
Robin Simsa
VERIGRAFT AB
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Decellularization
Tissue Engineering
Blood Vessel
<p>Decellularization of blood vessels is a promising approach to generate native biomaterials for replacement of diseased vessels. The decellularization process affects the mechanical properties of the vascular graft and thus can have a negative impact for in vivo functionality. The aim of this study was to determine how detergents under different fluid dynamics affects decellularization efficacy and mechanical properties of the vascular graft. We applied a protocol utilizing 1% TritonX, 1% Tributyl phosphate (TnBP) and DNase on porcine vena cava. The detergents were applied to the vessels under different conditions; static, agitation and perfusion with 3 different perfusion rates (25, 100 and 400 mL/min). The decellularized grafts were analyzed with histological, immunohistochemical and mechanical tests. We found that decellularization efficacy was equal in all groups, however the luminal ultrastructure of the static group showed remnant cell debris and the 400 mL/min perfusion group showed local damage and tearing of the luminal surface. The mechanical stiffness and maximum tensile strength were not influenced by the detergent application method. In conclusion, our results indicate that agitation or low-velocity perfusion with detergents are preferable methods for blood vessel decellularization.</p>
Zenodo
2019-08-05
info:eu-repo/semantics/article
3381095
user-fhtw
1579540851.686919
2610503
md5:fb03df1700fd2c635920cd3cda711db4
https://zenodo.org/records/3381096/files/Simsa et al - Effect of fluid dynamics.pdf
public
PLOS One
1-14
2019-08-05