Data - Effect of electrolytes as adjuvants in GFP and LPS partitioning on aqueous two-phase systems: 1. Polymer-polymer systems

Overview

The production of recombinant biopharmaceuticals is highly dependent of a proper choice of the downstream processing stages. Particularly, the purification that must ensure that all the endotoxins (lipopolysaccharide - LPS) are efficiently removed from the final product. This dataset contains the raw data and statistical analysis for the research entitled - "Effect of electrolytes as adjuvants in GFP and LPS partitioning on aqueous two-phase systems: 1. Polymer-polymer systems". 

Info

ANOVA_Turkey_Sub.R <- code for ANOVA analysis in R statistic 3.3.3    
glm.R <- code for GLM analysis in R statistic 3.3.3
K&REC_LPS_PEG_NaPA.xlsx <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) and recover (REC) for ANOVA analysis
K&REC_LPS_PEG_NaPA_K.docx <- File with ANOVA result of partition coefficient (K) for GFP
K&REC_LPS_PEG_NaPA_REC.docx <- File with ANOVA result of recover (REC) for GFP         
K_GFP_Pol_005.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in 0.05M salt assays    
K_GFP_Pol_005.doc <- File with GLM analysis of GFP partition coefficient (K) in 0.05M salt assays  
K_GFP_Pol_005_QQ.png <- Residual quantile plot of GLM analysis for partition coefficient (K) in 0.05M salt assays  
K_GFP_Pol_025.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in 0.25M salt assays  
K_GFP_Pol_025.doc  <- File with GLM analysis of GFP partition coefficient (K) in 0.25M salt assays  
K_GFP_Pol_025_QQ.png <- Residual quantile plot of GLM analysis for partition coefficient (K) in 0.25M salt assays         
REC_GFP_Pol_005.csv <- File with raw values organized in a spreadsheet of GFP recover (REC) for GLM analysis in 0.05M salt assays         
REC_GFP_Pol_005.doc  <- File with GLM analysis of GFP recover (REC) in 0.05M salt assays    
REC_GFP_Pol_005_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in 0.05M salt assays   
REC_GFP_Pol_025.csv <- File with raw values organized in a spreadsheet of GFP recover (REC) for GLM analysis in 0.25M salt assays          
REC_GFP_Pol_025.doc  <- File with GLM analysis of GFP recover (REC) in 0.25M salt assays 
REC_GFP_Pol_025_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in 0.25M salt assays        
REM_LPS_PEG_NaPA.docx  <- File with ANOVA result of LPS removal          
REM_LPS_PEG_NaPA.xlsx <- File with raw values organized in a spreadsheet of LPS removal for ANOVA analysis
Stability_GFP_PEG_NaPA.docx <- File with ANOVA result of GFP stability
Stability_GFP_PEG_NaPA.xlsx <- File with raw values organized in a spreadsheet of GFP stability results for ANOVA analysis

REM_LPS_Pol_005.csv  <- File with raw values organized in a spreadsheet of LPS removal (REM) for GLM analysis in 0.05M salt assays         
REM_LPS_Pol_005.doc  <- File with GLM analysis of LPS removal (REM) in 0.05M salt assays    
REM_LPS_Pol_005_QQ.png <- Residual quantile plot of GLM analysis of LPS removal (REM) in 0.05M salt assays  
REM_LPS_Pol_025.csv  <- File with raw values organized in a spreadsheet of LPS removal (REM) for GLM analysis in 0.25M salt assays         
REM_LPS_Pol_025.doc   <- File with GLM analysis of LPS removal (REM) in 0.25M salt assays    
REM_LPS_Pol_025_QQ.png <- Residual quantile plot of GLM analysis of LPS removal (REM) in 0.25M salt assays

K_GFP_Pol_025_NaCl_Li2SO4.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in 0.25M salt assays comparing NaCl and Li2SO4 effect 
K_GFP_Pol_025_NaCl_Li2SO4.doc  <- File with GLM analysis of GFP partition coefficient (K) in 0.25M salt assays comparing NaCl and Li2SO4 effect 
K_GFP_Pol_025_NaCl_Li2SO4_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in 0.25M salt assays comparing NaCl and Li2SO4 effect   

REM_LPS_Pol_KI_0.05_vs_0.25.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in KI assays comparing salt concentration effect  
REM_LPS_Pol_KI_0.05_vs_0.25.doc <- File with GLM analysis of GFP partition coefficient (K) in KI assays comparing salt concentration effect
REM_LPS_Pol_KI_0.05_vs_0.25_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in KI assays comparing salt concentration effect
REM_LPS_Pol_KNO3_0.05_vs_0.25.csv  <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in KNO3 assays comparing salt concentration effect  
REM_LPS_Pol_KNO3_0.05_vs_0.25.doc <- File with GLM analysis of GFP partition coefficient (K) in KNO3 assays comparing salt concentration effect
REM_LPS_Pol_KNO3_0.05_vs_0.25_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in KNO3 assays comparing salt concentration effect
REM_LPS_Pol_Li2SO4_0.05_vs_0.25.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in Li2SO4 assays comparing salt concentration effect  
REM_LPS_Pol_Li2SO4_0.05_vs_0.25.doc <- File with GLM analysis of GFP partition coefficient (K) in Li2SO4 assays comparing salt concentration effect
REM_LPS_Pol_Li2SO4_0.05_vs_0.25_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in Li2SO4 assays comparing salt concentration effect
REM_LPS_Pol_NaCl_0.05_vs_0.25.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in NaCl assays comparing salt concentration effect  
REM_LPS_Pol_NaCl_0.05_vs_0.25.doc <- File with GLM analysis of GFP partition coefficient (K) in NaCl assays comparing salt concentration effect 
REM_LPS_Pol_NaCl_0.05_vs_0.25_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in NaCl assays comparing salt concentration effect

Annotation

12/12 - Concentration of 12% of each polymer PEG/NaPA

16/16 - Concentration of 16% of each polymer PEG/NaPA

P/N -  PEG/NaPA

10e4, 10e5, 10e6 - Concentration of LPS in scientific notation - 10000, 100000, 100000 EU/mL

poly - Polymer

salt - Salt concentration in the assay

tsalt - Type of salt in the assay (NaCl, KNO3, KI and Li2SO4)

lps - lipopolysaccharide

K - GFP partition coefficient

REM - LPS removal

REC - GFP recover

wo_salt - Assay without salt addition

Acknowledgements

The authors are grateful for financial support from FAPESP (São Paulo Research Foundation, Brazil) through the following projects: 2005/60159-7; 2007/51978-0; 2014/16424-7; and 2014/19793-3. The authors also acknowledge the support from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil) through the process #0366/09-9 and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil).

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