Alterations of the Gut Microbiota and Metabolomics Associated with the Different Growth Performances of Macrobrachium rosenbergii Families

Figure S1: Box plots of alpha-diversity show differences among the three groups. (A) Chao1 index. (B) ACE index. (C) Observed species index. (D) Shannon index. (E) Simpson index. (F) PD whole tree index. The significance of any difference among the three groups is determined by Tukey's test, * indicates significant (p < 0.05), ** indicates highly significant (p < 0.01). H, high growth performance level; M, medium growth performance level; L, low growth performance level; Figure S2: The relative abundance of gut microbiota of GFP families with three growth performance groups at TOP10 of phylum (A) and TOP30 of genus (B) levels. H, high growth performance level; M, medium growth performance level; L, low growth performance level; Figure S3: Heatmap of bacterial communities for the 10 top differential abundance phyla (A), 30 top differential abundance genera (B), and the 20 top differential abundance species (C) among the three groups. H, high growth performance level; M, medium growth performance level; L, low growth performance level; Figure S4: Heatmaps of the differential abundance of metabolites in ESI⁺ (A) and ESI⁻ (B) scan modes between H and L groups. H, high growth performance level; L, low growth performance level; Figure S5: KEGG enrichment analysis of differential metabolites in different ion scan modes. (A) L vs. H in ESI⁺ mode. (B) L vs. H in ESI⁻ mode. (C) L vs. M in ESI⁺ mode. (D) L vs. M in ESI⁻ mode. (E) M vs. H in ESI⁺ mode. (F) M vs. H in ESI⁻ mode. H, high growth performance level; M, medium growth performance level; L, low growth performance level; Table S1: The information of OTUs identified in this study; Table S2: The relative abundance of gut microbiota by phylum, family, genus, and species; Table S3: The list of metabolites identified in this study.